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anti-ampk-α (ampkα; #5831, which recognizes both α1 and α2 isoforms)  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti-ampk-α (ampkα; #5831, which recognizes both α1 and α2 isoforms)
    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 <t>/AMPK</t> ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (IPEX or Sedentary), G P < 0.05; IPEX versus Sedentary within the same AAV group (shRNA-GS1 or shRNA-Scr), E P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.
    Anti Ampk α (Ampkα; #5831, Which Recognizes Both α1 And α2 Isoforms), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ampk+%CE%B11+%CE%B12/ampk+antibody/pmc10826964-50-26-61
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    anti-ampk-α (ampkα; #5831, which recognizes both α1 and α2 isoforms) - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise"

    Article Title: A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0295964

    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (IPEX or Sedentary), G P < 0.05; IPEX versus Sedentary within the same AAV group (shRNA-GS1 or shRNA-Scr), E P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.
    Figure Legend Snippet: (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (IPEX or Sedentary), G P < 0.05; IPEX versus Sedentary within the same AAV group (shRNA-GS1 or shRNA-Scr), E P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.

    Techniques Used: Western Blot, shRNA

    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (5hPEX or 5hSed) and diet group (refed or not refed), G P < 0.05; 5hPEX versus 5hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 5h-RF versus 5h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (5hPEX or 5hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6-12/group for all the immunoblots and glycogen.
    Figure Legend Snippet: (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (5hPEX or 5hSed) and diet group (refed or not refed), G P < 0.05; 5hPEX versus 5hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 5h-RF versus 5h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (5hPEX or 5hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6-12/group for all the immunoblots and glycogen.

    Techniques Used: Western Blot, shRNA

    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (9hPEX or 9hSed) and diet group (refed or not refed, G P < 0.05; 9hPEX versus 9hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 9h-RF versus 9h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (9hPEX or 9hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.
    Figure Legend Snippet: (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (9hPEX or 9hSed) and diet group (refed or not refed, G P < 0.05; 9hPEX versus 9hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 9h-RF versus 9h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (9hPEX or 9hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.

    Techniques Used: Western Blot, shRNA

    Related Articles

    Western Blot:

    Article Title: Hepatic E4BP4 induction promotes lipid accumulation by suppressing AMPK signaling in response to chemical or diet‐induced ER stress
    Article Snippet: .. Western blot analysis was performed using the following primary antibodies: anti-GAPDH (Santa Cruz, sc-25778), anti-Lamin A/C (Santa Cruz, sc20681), anti-HSP90 (Santa Cruz, sc-13119), anti-CHOP (Santa Cruz, sc-575), anti-GRP78 (Santa Cruz, sc-13968), anti-E4BP4 (Santa Cruz, sc-28203; Cell Signaling, 14312S; DSHB, PCRP-NFIL3-2B5), anti-AMPK-pT172 (Cell Signaling, 2531S), anti-AMPK α1/α2 (Cell Signaling, 5831S), antiAMPK-β1 (Santa Cruz, sc-100357), anti-AMPK-γ1 (Santa Cruz, sc-19138), anti-β-tubulin(Sigma,T5201), and anti-FLAG (Sigma, A8592). .. Total cellular RNA extraction was performed with TRIzol (Invitrogen) and chloroform. cDNA was synthesized with the Verso cDNA kit (Thermo Fisher Scientific) and subjected to qPCR analysis with Radiant Green 2X qPCR Mix (Alkali Scientific) on an ABI 7900 HT thermal cycler (Applied Biosystems).

    Article Title: Hepatic E4BP4 induction promotes lipid accumulation by suppressing AMPK signaling in response to chemical or diet-induced ER stress
    Article Snippet: .. The nuclear fraction was then collected after centrifugation at 13000 x rpm for 10 min. Western blot analysis was performed using the following primary antibodies: anti-GAPDH (Santa Cruz, sc-25778), anti-Lamin A/C (Santa Cruz, sc-20681), anti-HSP90 (Santa Cruz, sc-13119), anti-CHOP (Santa Cruz, sc-575), anti-GRP78 (Santa Cruz, sc-13968), anti-E4BP4 (Santa Cruz, sc-28203; Cell Signaling, 14312S; DSHB, PCRP-NFIL3-2B5), anti-AMPK-p T172 (Cell Signaling, 2531S), anti-AMPK α1/α2 (Cell Signaling, 5831S), anti-AMPK-β1 (Santa Cruz, sc-100357), anti-AMPK-γ1 (Santa Cruz, sc-19138), anti-β-tubulin(Sigma,T5201), and anti-FLAG (Sigma, A8592). .. Total cellular RNA extraction was performed with TRIzol (Invitrogen) and chloroform. cDNA was synthesized with the Verso cDNA kit (ThermoFisher Scientific) and subjected to qPCR analysis with RadiantTM Green 2X qPCR Mix (Alkali Scientific) on an ABI 7900 HT thermal cycler (Applied Biosystems).

    Centrifugation:

    Article Title: Hepatic E4BP4 induction promotes lipid accumulation by suppressing AMPK signaling in response to chemical or diet-induced ER stress
    Article Snippet: .. The nuclear fraction was then collected after centrifugation at 13000 x rpm for 10 min. Western blot analysis was performed using the following primary antibodies: anti-GAPDH (Santa Cruz, sc-25778), anti-Lamin A/C (Santa Cruz, sc-20681), anti-HSP90 (Santa Cruz, sc-13119), anti-CHOP (Santa Cruz, sc-575), anti-GRP78 (Santa Cruz, sc-13968), anti-E4BP4 (Santa Cruz, sc-28203; Cell Signaling, 14312S; DSHB, PCRP-NFIL3-2B5), anti-AMPK-p T172 (Cell Signaling, 2531S), anti-AMPK α1/α2 (Cell Signaling, 5831S), anti-AMPK-β1 (Santa Cruz, sc-100357), anti-AMPK-γ1 (Santa Cruz, sc-19138), anti-β-tubulin(Sigma,T5201), and anti-FLAG (Sigma, A8592). .. Total cellular RNA extraction was performed with TRIzol (Invitrogen) and chloroform. cDNA was synthesized with the Verso cDNA kit (ThermoFisher Scientific) and subjected to qPCR analysis with RadiantTM Green 2X qPCR Mix (Alkali Scientific) on an ABI 7900 HT thermal cycler (Applied Biosystems).

    other:

    Article Title: Hepatic E4BP4 induction promotes lipid accumulation by suppressing AMPK signaling in response to chemical or diet-induced ER stress
    Article Snippet: Then, the slides were stained with Picro-Sirius Red Collagen Stain Kit (Sigma, Direct Red 80, 365548; FAST Green, 7252) or Masson’s Trichrome Stain Kit (Sigma, HT15-1KT) according to the user’s manuals and observed under microscope.



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    Image Search Results


    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (IPEX or Sedentary), G P < 0.05; IPEX versus Sedentary within the same AAV group (shRNA-GS1 or shRNA-Scr), E P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.

    Journal: PLOS ONE

    Article Title: A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise

    doi: 10.1371/journal.pone.0295964

    Figure Lengend Snippet: (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (IPEX or Sedentary), G P < 0.05; IPEX versus Sedentary within the same AAV group (shRNA-GS1 or shRNA-Scr), E P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.

    Article Snippet: Anti-glycogen synthase I (GS1; #3893), anti-hexokinase II (HKII; #2867), Anti-phospho AMPKα Thr 172 (pAMPKα Thr172 ; #50081, which recognizes phosphorylation on both α1 and α2 isoforms), anti-AMPK-α (AMPKα; #5831, which recognizes both α1 and α2 isoforms), anti-acetyl CoA carboxylase (ACC; #3676), anti-phospho ACC Ser79/212 (pACC Ser79/212 ; #3661), anti-TBC1D1 (TBC1D1; #4629), and anti-rabbit IgG horseradish peroxidase (HRP) conjugate (#7074) were from Cell Signaling Technology (Danvers, MA).

    Techniques: Western Blot, shRNA

    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (5hPEX or 5hSed) and diet group (refed or not refed), G P < 0.05; 5hPEX versus 5hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 5h-RF versus 5h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (5hPEX or 5hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6-12/group for all the immunoblots and glycogen.

    Journal: PLOS ONE

    Article Title: A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise

    doi: 10.1371/journal.pone.0295964

    Figure Lengend Snippet: (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (5hPEX or 5hSed) and diet group (refed or not refed), G P < 0.05; 5hPEX versus 5hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 5h-RF versus 5h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (5hPEX or 5hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6-12/group for all the immunoblots and glycogen.

    Article Snippet: Anti-glycogen synthase I (GS1; #3893), anti-hexokinase II (HKII; #2867), Anti-phospho AMPKα Thr 172 (pAMPKα Thr172 ; #50081, which recognizes phosphorylation on both α1 and α2 isoforms), anti-AMPK-α (AMPKα; #5831, which recognizes both α1 and α2 isoforms), anti-acetyl CoA carboxylase (ACC; #3676), anti-phospho ACC Ser79/212 (pACC Ser79/212 ; #3661), anti-TBC1D1 (TBC1D1; #4629), and anti-rabbit IgG horseradish peroxidase (HRP) conjugate (#7074) were from Cell Signaling Technology (Danvers, MA).

    Techniques: Western Blot, shRNA

    (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (9hPEX or 9hSed) and diet group (refed or not refed, G P < 0.05; 9hPEX versus 9hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 9h-RF versus 9h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (9hPEX or 9hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.

    Journal: PLOS ONE

    Article Title: A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise

    doi: 10.1371/journal.pone.0295964

    Figure Lengend Snippet: (A) Glycogen synthase abundance. (B) Glycogen. (C) HKII abundance. (D) GLUT4 abundance. (E) PGC1α abundance. (F) PDK4 abundance. (G) Representative immunoblots. (H) pAMPK Thr172 /AMPK ratio. (I) pACC1/2 Ser79/212 /ACC1/2 ratio. (J) pTBC1D1 Ser237 / TBC1D1 ratio. (K) Representative immunoblots. (L) PYGM abundance. (M) AGL abundance. (N) GBE1 abundance. (O) Representative immunoblots. shRNA-GS1 versus shRNA-Scr within the same exercise group (9hPEX or 9hSed) and diet group (refed or not refed, G P < 0.05; 9hPEX versus 9hSed within the same AAV (shRNA-GS1 or shRNA-Scr) and diet group (refed or not refed), E P < 0.05; 9h-RF versus 9h-NRF within the same AAV (shRNA-GS1 or shRNA-Scr) and exercise group (9hPEX or 9hSed), D P < 0.05. Comparisons between the two groups were analyzed by a multilevel mixed-effects linear regression analysis. Values are means ± SD; n = 6/group for all the immunoblots and glycogen.

    Article Snippet: Anti-glycogen synthase I (GS1; #3893), anti-hexokinase II (HKII; #2867), Anti-phospho AMPKα Thr 172 (pAMPKα Thr172 ; #50081, which recognizes phosphorylation on both α1 and α2 isoforms), anti-AMPK-α (AMPKα; #5831, which recognizes both α1 and α2 isoforms), anti-acetyl CoA carboxylase (ACC; #3676), anti-phospho ACC Ser79/212 (pACC Ser79/212 ; #3661), anti-TBC1D1 (TBC1D1; #4629), and anti-rabbit IgG horseradish peroxidase (HRP) conjugate (#7074) were from Cell Signaling Technology (Danvers, MA).

    Techniques: Western Blot, shRNA

    Protein expressions of AMPK- α1 ( A ) and AMPK-α2 ( B ) in skeletal muscle of IGT subjects. ( C ) Representative Western blots, using anti-AMKP-α1 and AMPK-α2 and beta-actin antibodies from skeletal muscle biopsies of non-responders and responders at baseline (0) and after the 2-year intervention.

    Journal: International Journal of Environmental Research and Public Health

    Article Title: Improved Aerobic Capacity and Adipokine Profile Together with Weight Loss Improve Glycemic Control without Changes in Skeletal Muscle GLUT-4 Gene Expression in Middle-Aged Subjects with Impaired Glucose Tolerance

    doi: 10.3390/ijerph19148327

    Figure Lengend Snippet: Protein expressions of AMPK- α1 ( A ) and AMPK-α2 ( B ) in skeletal muscle of IGT subjects. ( C ) Representative Western blots, using anti-AMKP-α1 and AMPK-α2 and beta-actin antibodies from skeletal muscle biopsies of non-responders and responders at baseline (0) and after the 2-year intervention.

    Article Snippet: The blots were incubated overnight at +4 °C with the following antibodies: goat polyclonal anti-AMPK-α1 and -AMPK-α2 (Santa Cruz, CA, USA).

    Techniques: Western Blot